Publications

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Walczak CE, Ohi R. Microtubule depolymerizing kinesins PMCID: PMC Journal In Process. Annual review of cell and developmental biology. Forthcoming;

Lessard DV, Zinder OJ, Hotta T, Verhey KJ, Ryoma O, Berger CL. Regulation of KIF1A motility via polyglutamylation of tubulin C-terminal tails. Journal of Biol. Chem. Forthcoming; doi: 10.1101/410860.

Malaby HLH, Dumas ME, Ohi R, Stumpff J. Kinesin-binding protein ensures accurate chromosome segregation by buffering KIF18A and KIF15. J Cell Biol. 2019 Apr 1;218(4):1218-1234. doi: 10.1083/jcb.201806195. Epub 2019 Feb 1. PubMed PMID: 30709852; PubMed Central PMCID: PMC6446846.

Fonseca CL, Malaby HLH, Sepaniac LA, Martin W, Byers C, Czechanski A, Messinger D, Tang M, Ohi R, Reinholdt LG, Stumpff J. Mitotic chromosome alignment ensures mitotic fidelity by promoting interchromosomal compaction during anaphase. J Cell Biol. 2019 Apr 1;218(4):1148-1163. doi: 10.1083/jcb.201807228. Epub 2019 Feb 7. PubMed PMID: 30733233; PubMed Central PMCID: PMC6446859.

Dumas ME, Chen GY, Kendrick ND, Xu G, Larsen SD, Jana S, Waterson AG, Bauer JA, Hancock W, Sulikowski GA, Ohi R. Dual inhibition of Kif15 by oxindole and quinazolinedione chemical probes. Bioorg Med Chem Lett. 2019 Jan 15;29(2):148-154. doi: 10.1016/j.bmcl.2018.12.008. Epub 2018 Dec 4. PubMed PMID: 30528696; PubMed Central PMCID: PMC6681659.

Ohi R, Verhey KJ. Promoting Diversity in the Cytoskeleton through STEM-Structural Transitions and Energetics of Microtubule Subunits. Dev Cell. 2018 Oct 22;47(2):137-139. doi: 10.1016/j.devcel.2018.10.008. PubMed PMID: 30352173; PubMed Central PMCID: PMC6396305.

Reinemann DN, Norris SR, Ohi R, Lang MJ. Processive Kinesin-14 HSET Exhibits Directional Flexibility Depending on Motor Traffic. Curr Biol. 2018 Jul 23;28(14):2356-2362.e5. doi: 10.1016/j.cub.2018.06.055. Epub 2018 Jul 12. PubMed PMID: 30017484.

Norris SR, Jung S, Singh P, Strothman CE, Erwin AL, Ohi MD, Zanic M, Ohi R. Microtubule minus-end aster organization is driven by processive HSET-tubulin clusters. Nat Commun. 2018 Jul 9;9(1):2659. doi: 10.1038/s41467-018-04991-2. PubMed PMID: 29985404; PubMed Central PMCID: PMC6037785.

Chiang YC, Park IY, Terzo EA, Tripathi DN, Mason FM, Fahey CC, Karki M, Shuster CB, Sohn BH, Chowdhury P, Powell RT, Ohi R, Tsai YS, de Cubas AA, Khan A, Davis IJ, Strahl BD, Parker JS, Dere R, Walker CL, Rathmell WK. SETD2 Haploinsufficiency for Microtubule Methylation Is an Early Driver of Genomic Instability in Renal Cell Carcinoma. Cancer Res. 2018 Jun 15;78(12):3135-3146. doi: 10.1158/0008-5472.CAN-17-3460. Epub 2018 May 3. PubMed PMID: 29724720; PubMed Central PMCID: PMC6004258.

Landino J, Norris SR, Li M, Ballister ER, Lampson MA, Ohi R. Two mechanisms coordinate the recruitment of the chromosomal passenger complex to the plane of cell division. Mol Biol Cell. 2017 Dec 1;28(25):3634-3646. doi: 10.1091/mbc.E17-06-0399. Epub 2017 Sep 27. PubMed PMID: 28954866; PubMed Central PMCID: PMC5706991.

Reinemann DN, Sturgill EG, Das DK, Degen MS, Vörös Z, Hwang W, Ohi R, Lang MJ. Collective Force Regulation in Anti-parallel Microtubule Gliding by Dimeric Kif15 Kinesin Motors. Curr Biol. 2017 Sep 25;27(18):2810-2820.e6. doi: 10.1016/j.cub.2017.08.018. Epub 2017 Sep 14. PubMed PMID: 28918951; PubMed Central PMCID: PMC5909953.

Norris SR, Ohi R. Cell Division: Centrosomes Have Separation Anxiety. Curr Biol. 2017 Jun 19;27(12):R601-R603. doi: 10.1016/j.cub.2017.04.050. PubMed PMID: 28633031.

Chen GY, Kang YJ, Gayek AS, Youyen W, Tüzel E, Ohi R, Hancock WO. Eg5 Inhibitors Have Contrasting Effects on Microtubule Stability and Metaphase Spindle Integrity. ACS Chem Biol. 2017 Apr 21;12(4):1038-1046. doi: 10.1021/acschembio.6b01040. Epub 2017 Feb 22. PubMed PMID: 28165699; PubMed Central PMCID: PMC5515541.

Dumas ME, Sturgill EG, Ohi R. Resistance is not futile: Surviving Eg5 inhibition. Cell Cycle. 2016 Nov;15(21):2845-2847. doi: 10.1080/15384101.2016.1204864. Epub 2016 Jun 29. PubMed PMID: 27356105; PubMed Central PMCID: PMC5105924.

Pfaltzgraff ER, Roth GM, Miller PM, Gintzig AG, Ohi R, Bader DM. Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities. Mol Biol Cell. 2016 Jul 1;27(13):1990-9. doi: 10.1091/mbc.E15-12-0848. Epub 2016 May 4. PubMed PMID: 27146114; PubMed Central PMCID: PMC4927273.

Gayek AS, Ohi R. CDK-1 Inhibition in G2 Stabilizes Kinetochore-Microtubules in the following Mitosis. PLoS One. 2016;11(6):e0157491. doi: 10.1371/journal.pone.0157491. eCollection 2016. PubMed PMID: 27281342; PubMed Central PMCID: PMC4900577.

Sturgill EG, Norris SR, Guo Y, Ohi R. Kinesin-5 inhibitor resistance is driven by kinesin-12. J Cell Biol. 2016 Apr 25;213(2):213-27. doi: 10.1083/jcb.201507036. Epub 2016 Apr 18. PubMed PMID: 27091450; PubMed Central PMCID: PMC5084272.

Ohi R, Zanic M. Ahead of the Curve: New Insights into Microtubule Dynamics. F1000Res. 2016;5. doi: 10.12688/f1000research.7439.1. eCollection 2016. Review. PubMed PMID: 26998244; PubMed Central PMCID: PMC4786905.

Fenix AM, Taneja N, Buttler CA, Lewis J, Van Engelenburg SB, Ohi R, Burnette DT. Expansion and concatenation of non-muscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis. Mol Biol Cell. 2016 Mar 9;. doi: 10.1091/mbc.E15-10-0725. [Epub ahead of print] PubMed PMID: 26960797; PubMed Central PMCID: PMC4850034.

Landino J, Ohi R. The Timing of Midzone Stabilization during Cytokinesis Depends on Myosin II Activity and an Interaction between INCENP and Actin. Curr Biol. 2016 Mar 7;26(5):698-706. doi: 10.1016/j.cub.2016.01.018. Epub 2016 Feb 18. PubMed PMID: 26898472; PubMed Central PMCID: PMC4783181.

Shin Y, Du Y, Collier SE, Ohi MD, Lang MJ, Ohi R. Biased Brownian motion as a mechanism to facilitate nanometer-scale exploration of the microtubule plus end by a kinesin-8. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):E3826-35. doi: 10.1073/pnas.1500272112. Epub 2015 Jul 6. PubMed PMID: 26150501; PubMed Central PMCID: PMC4517267.

Gayek AS, Ohi R. Fanning the flames of CIN. Cell Cycle. 2015;14(16):2560. doi: 10.1080/15384101.2015.1018053. PubMed PMID: 25714682; PubMed Central PMCID: PMC5242322.

Collier SE, Voehler M, Peng D, Ohi R, Gould KL, Reiter NJ, Ohi MD. Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Biochemistry. 2014 Oct 21;53(41):6439-51. doi: 10.1021/bi5008639. Epub 2014 Oct 8. PubMed PMID: 25263959; PubMed Central PMCID: PMC4204884.

Sturgill EG, Das DK, Takizawa Y, Shin Y, Collier SE, Ohi MD, Hwang W, Lang MJ, Ohi R. Kinesin-12 Kif15 targets kinetochore fibers through an intrinsic two-step mechanism. Curr Biol. 2014 Oct 6;24(19):2307-13. doi: 10.1016/j.cub.2014.08.022. Epub 2014 Sep 25. PubMed PMID: 25264249; PubMed Central PMCID: PMC4207087.

Gayek AS, Ohi R. Kinetochore-microtubule stability governs the metaphase requirement for Eg5. Mol Biol Cell. 2014 Jul 1;25(13):2051-60. doi: 10.1091/mbc.E14-03-0785. Epub 2014 May 7. PubMed PMID: 24807901; PubMed Central PMCID: PMC4072578.

Sturgill EG, Ohi R. Microtubule-regulating kinesins. Curr Biol. 2013 Nov 4;23(21):R946-8. doi: 10.1016/j.cub.2013.08.012. PubMed PMID: 24200317.

Sturgill EG, Ohi R. Kinesin-12 differentially affects spindle assembly depending on its microtubule substrate. Curr Biol. 2013 Jul 22;23(14):1280-90. doi: 10.1016/j.cub.2013.05.043. Epub 2013 Jun 20. PubMed PMID: 23791727; PubMed Central PMCID: PMC3725213.

Walczak CE, Gayek S, Ohi R. Microtubule-depolymerizing kinesins. Annu Rev Cell Dev Biol. 2013;29:417-41. doi: 10.1146/annurev-cellbio-101512-122345. Epub 2013 Jul 17. Review. PubMed PMID: 23875646.

Su X, Ohi R, Pellman D. Move in for the kill: motile microtubule regulators. Trends Cell Biol. 2012 Nov;22(11):567-75. doi: 10.1016/j.tcb.2012.08.003. Epub 2012 Sep 6. Review. PubMed PMID: 22959403; PubMed Central PMCID: PMC3482944.

Ohi R, Wordeman L. Kinesins as Microtubule Disassembly Enzymes . In: Encyclopedia of Biological Chemistry. Parent C, Coulombe P, editors. TBD (in press): Elsevier Press; 2012.

Chen JS, Lu LX, Ohi MD, Creamer KM, English C, Partridge JF, Ohi R, Gould KL. Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol. 2011 Nov 14;195(4):583-93. doi: 10.1083/jcb.201105074. Epub 2011 Nov 7. PubMed PMID: 22065639; PubMed Central PMCID: PMC3257533.

Archuleta TL, Du Y, English CA, Lory S, Lesser C, Ohi MD, Ohi R, Spiller BW. The Chlamydia effector chlamydial outer protein N (CopN) sequesters tubulin and prevents microtubule assembly. J Biol Chem. 2011 Sep 30;286(39):33992-8. doi: 10.1074/jbc.M111.258426. Epub 2011 Aug 13. PubMed PMID: 21841198; PubMed Central PMCID: PMC3190796.

Stumpff J, Du Y, English CA, Maliga Z, Wagenbach M, Asbury CL, Wordeman L, Ohi R. A tethering mechanism controls the processivity and kinetochore-microtubule plus-end enrichment of the kinesin-8 Kif18A. Mol Cell. 2011 Sep 2;43(5):764-75. doi: 10.1016/j.molcel.2011.07.022. PubMed PMID: 21884977; PubMed Central PMCID: PMC3172727.

Ohi R. Kip3-ing kinetochores clustered. Cell Cycle. 2010 Jul 1;9(13):2497. doi: 10.4161/cc.9.13.12274. PubMed PMID: 20647749; PubMed Central PMCID: PMC3233490.

Du Y, English CA, Ohi R. The kinesin-8 Kif18A dampens microtubule plus-end dynamics. Curr Biol. 2010 Feb 23;20(4):374-80. doi: 10.1016/j.cub.2009.12.049. Epub 2010 Feb 11. PubMed PMID: 20153196.

Needleman DJ, Groen A, Ohi R, Maresca T, Mirny L, Mitchison T. Fast microtubule dynamics in meiotic spindles measured by single molecule imaging: evidence that the spindle environment does not stabilize microtubules. Mol Biol Cell. 2010 Jan 15;21(2):323-33. doi: 10.1091/mbc.e09-09-0816. Epub 2009 Nov 25. PubMed PMID: 19940016; PubMed Central PMCID: PMC2808228.

Ohi R. Kip3-ing kinetochores clustered. Cell Cycle 9(13): 2496. Invited Review. 2010. .

Maresca TJ, Groen AC, Gatlin JC, Ohi R, Mitchison TJ, Salmon ED. Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity. Curr Biol. 2009 Jul 28;19(14):1210-5. doi: 10.1016/j.cub.2009.05.061. Epub 2009 Jun 18. PubMed PMID: 19540121; PubMed Central PMCID: PMC2752711.

Germane KL, Ohi R, Goldberg MB, Spiller BW. Structural and functional studies indicate that Shigella VirA is not a protease and does not directly destabilize microtubules. Biochemistry. 2008 Sep 30;47(39):10241-3. doi: 10.1021/bi801533k. Epub 2008 Sep 3. PubMed PMID: 18763811; PubMed Central PMCID: PMC4096815.

Efimov A, Schiefermeier N, Grigoriev I, Ohi R, Brown MC, Turner CE, Small JV, Kaverina I. Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites. J Cell Sci. 2008 Jan 15;121(Pt 2):196-204. doi: 10.1242/jcs.012666. PubMed PMID: 18187451; PubMed Central PMCID: PMC3164837.

Ohi R, Burbank K, Liu Q, Mitchison TJ. Nonredundant functions of Kinesin-13s during meiotic spindle assembly. Curr Biol. 2007 Jun 5;17(11):953-9. doi: 10.1016/j.cub.2007.04.057. Epub 2007 May 17. PubMed PMID: 17509883.

Coughlin M, Brieher WM, Ohi R. Cell-free extract systems and the cytoskeleton: preparation of biochemical experiments for transmission electron microscopy. Methods Mol Biol. 2007;369:199-212. doi: 10.1007/978-1-59745-294-6_10. PubMed PMID: 17656752.

Mitchison TJ, Maddox P, Groen A, Cameron L, Perlman Z, Ohi R, Desai A, Salmon ED, Kapoor TM. Bipolarization and poleward flux correlate during Xenopus extract spindle assembly. Mol Biol Cell. 2004 Dec;15(12):5603-15. doi: 10.1091/mbc.e04-05-0440. Epub 2004 Sep 22. PubMed PMID: 15385629; PubMed Central PMCID: PMC532038.

Groen AC, Cameron LA, Coughlin M, Miyamoto DT, Mitchison TJ, Ohi R. XRHAMM functions in ran-dependent microtubule nucleation and pole formation during anastral spindle assembly. Curr Biol. 2004 Oct 26;14(20):1801-11. doi: 10.1016/j.cub.2004.10.002. PubMed PMID: 15498487.

Sampath SC, Ohi R, Leismann O, Salic A, Pozniakovski A, Funabiki H. The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly. Cell. 2004 Jul 23;118(2):187-202. doi: 10.1016/j.cell.2004.06.026. PubMed PMID: 15260989.

Ohi R, Sapra T, Howard J, Mitchison TJ. Differentiation of cytoplasmic and meiotic spindle assembly MCAK functions by Aurora B-dependent phosphorylation. Mol Biol Cell. 2004 Jun;15(6):2895-906. doi: 10.1091/mbc.e04-02-0082. Epub 2004 Apr 2. PubMed PMID: 15064354; PubMed Central PMCID: PMC420112.

Ohi R, Coughlin ML, Lane WS, Mitchison TJ. An inner centromere protein that stimulates the microtubule depolymerizing activity of a KinI kinesin. Dev Cell. 2003 Aug;5(2):309-21. doi: 10.1016/s1534-5807(03)00229-6. PubMed PMID: 12919681.

Burns CG, Ohi R, Mehta S, O’Toole ET, Winey M, Clark TA, Sugnet CW, Ares M Jr, Gould KL. Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol. 2002 Feb;22(3):801-15. doi: 10.1128/mcb.22.3.801-815.2002. PubMed PMID: 11784857; PubMed Central PMCID: PMC133559.

Burns CG, Ohi R, Krainer AR, Gould KL. Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13789-94. doi: 10.1073/pnas.96.24.13789. PubMed PMID: 10570151; PubMed Central PMCID: PMC24143.

McDonald WH, Ohi R, Smelkova N, Frendewey D, Gould KL. Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing. Mol Cell Biol. 1999 Aug;19(8):5352-62. doi: 10.1128/mcb.19.8.5352. PubMed PMID: 10409726; PubMed Central PMCID: PMC84378.

Feoktistova A, McCollum D, Ohi R, Gould KL. Identification and characterization of Schizosaccharomyces pombe asp1(+), a gene that interacts with mutations in the Arp2/3 complex and actin. Genetics. 1999 Jul;152(3):895-908. PubMed PMID: 10388810; PubMed Central PMCID: PMC1460656.

Ohi R, Gould KL. Regulating the onset of mitosis. Curr Opin Cell Biol. 1999 Apr;11(2):267-73. doi: 10.1016/s0955-0674(99)80036-2. Review. PubMed PMID: 10209160.

Ohi R, Feoktistova A, McCann S, Valentine V, Look AT, Lipsick JS, Gould KL. Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes. Mol Cell Biol. 1998 Jul;18(7):4097-108. doi: 10.1128/mcb.18.7.4097. PubMed PMID: 9632794; PubMed Central PMCID: PMC108994.Ohi R, McCollum D, Hirani B, Den Haese GJ, Zhang X, Burke JD, Turner K, Gould KL. The Schizosaccharomyces pombe cdc5+ gene encodes an essential protein with homology to c-Myb. EMBO J. 1994 Jan 15;13(2):471-83. PubMed PMID: 8313892; PubMed Central PMCID: PMC394831.